Yeast mitochondrial Gln-tRNA is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS
نویسندگان
چکیده
Mathieu Frechin, Bruno Senger, Mélanie Brayé, Daniel Kern, Robert Pierre Martin, and Hubert Dominique Becker UPR 9002, ‘‘Architecture et Réactivité de l’ARN,’’ Université de Strasbourg, CNRS, Institut de Biologie Moléculaire et Cellulaire, F-67084 Strasbourg Cédex, France; UMR 7156, ‘‘Génétique Moléculaire, Génomique, Microbiologie,’’ Department of Molecular and Cellular Genetics, CNRS, Université de Strasbourg, 67084 Strasbourg, France
منابع مشابه
Yeast mitochondrial Gln-tRNA(Gln) is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS.
It is impossible to predict which pathway, direct glutaminylation of tRNA(Gln) or tRNA-dependent transamidation of glutamyl-tRNA(Gln), generates mitochondrial glutaminyl-tRNA(Gln) for protein synthesis in a given species. The report that yeast mitochondria import both cytosolic glutaminyl-tRNA synthetase and tRNA(Gln) has challenged the widespread use of the transamidation pathway in organelles...
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The yeast aminoacyl-tRNA synthetase (aaRS) complex is formed by the methionyl- and glutamyl-tRNA synthetases (MetRS and GluRS, respectively) and the tRNA aminoacylation cofactor Arc1p. It is considered an evolutionary intermediate between prokaryotic aaRS and the multi- aaRS complex found in higher eukaryotes. While a wealth of structural information is available on the enzymatic domains of sin...
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Yeast mitochondrial Gln-mtRNAGln is synthesized by the transamidation of mischarged Glu-mtRNAGln by a non-canonical heterotrimeric tRNA-dependent amidotransferase (AdT). The GatA and GatB subunits of the yeast AdT (GatFAB) are well conserved among bacteria and eukaryota, but the GatF subunit is a fungi-specific ortholog of the GatC subunit found in all other known heterotrimeric AdTs (GatCAB). ...
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